A method for the efficient evaluation of substrate-based cholinesterase imaging probes for Alzheimer’s disease

Cholinesterase (ChE) enzymes have been identified as diagnostic markers for Alzheimer disease (AD). Substrate-based probes synthesised to detect ChEs but they not detected changes in ChE distribution associated with AD pathology. Probes are typically screened using spectrophotometric methods pure enzyme specificity and kinetics. However, the biochemical properties of pathology altered. The present work was undertaken determine whether Karnovsky-Roots (KR) histochemical method could be used evaluate at site Thirty thioesters esters were evaluated kinetic KR methods. Spectrophotometric demonstrated all substrates, yet only a few provided staining brain method. Esters substrates interactions ChEs. These results suggest that may provide an efficient means screen compounds imaging AD-associated